A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
- We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-cis-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies.
Author: | Simone Stegmüller, Nadine Beißmann, Jonathan Isaak KremerORCiD, Denise Mehl, Christian Baumann, Elke RichlingORCiD |
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URN: | urn:nbn:de:hbz:386-kluedo-61797 |
ISSN: | 1420-3049 |
Parent Title (English): | Molecules |
Publisher: | MDPI |
Document Type: | Article |
Language of publication: | English |
Date of Publication (online): | 2020/11/03 |
Year of first Publication: | 2020 |
Publishing Institution: | Technische Universität Kaiserslautern |
Date of the Publication (Server): | 2021/01/14 |
Issue: | (2020) 25 |
Page Number: | 14 |
Source: | https://www.mdpi.com/1420-3049/25/21/5104 |
Faculties / Organisational entities: | Kaiserslautern - Fachbereich Chemie |
DDC-Cassification: | 5 Naturwissenschaften und Mathematik / 540 Chemie |
Collections: | Open-Access-Publikationsfonds |
Licence (German): | Zweitveröffentlichung |