Proximity channeling during cyanobacterialphycoerythrobilin synthesis
- Substrate channeling is a widespread mechanism in metabolic pathways to avoid decomposition of unstable intermediates, competing reactions, and to accelerate catalytic turnover. During the biosynthesis of light-harvesting phycobilins in cyanobacteria, two members of the ferredoxin-dependent bilin reductases are involved in the reduction of the open-chain tetrapyrrole biliverdin IXα to the pink pigment phycoerythrobilin. The first reaction is catalyzed by 15,16-dihydrobiliverdin:ferredoxin oxidoreductase and produces the unstable intermediate 15,16-dihydrobiliverdin (DHBV). This intermediate is subsequently converted by phycoerythrobilin:ferredoxin oxidoreductase to the final product phycoerythrobilin. Although substrate channeling has been postulated already a decade ago, detailed experimental evidence was missing. Using a new on-column assay employing immobilized enzyme in combination with UV-Vis and fluorescence spectroscopy revealed that both enzymes transiently interact and that transfer of the intermediate is facilitated by a significantly higher binding affinity of DHBV toward phycoerythrobilin:ferredoxin oxidoreductase. Concluding from the presented data, the intermediate DHBV is transferred via proximity channeling.
Verfasser*innenangaben: | Marco Aras, Volker Hartmann, Jana Hartmann, Marc M. Nowaczyk, Nicole Frankenberg-DinkelORCiD |
---|---|
URN: | urn:nbn:de:hbz:386-kluedo-79672 |
DOI: | https://doi.org/10.1111/febs.15003 |
ISSN: | 1742-4658 |
Titel des übergeordneten Werkes (Englisch): | The FEBS Journal |
Verlag: | Wiley |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache der Veröffentlichung: | Englisch |
Datum der Veröffentlichung (online): | 08.04.2024 |
Jahr der Erstveröffentlichung: | 2019 |
Veröffentlichende Institution: | Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau |
Datum der Publikation (Server): | 08.04.2024 |
Ausgabe / Heft: | 287/2 |
Seitenzahl: | 11 |
Erste Seite: | 284 |
Letzte Seite: | 294 |
Quelle: | https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15003 |
Fachbereiche / Organisatorische Einheiten: | Kaiserslautern - Fachbereich Biologie |
DDC-Sachgruppen: | 5 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie |
Sammlungen: | Open-Access-Publikationsfonds |
Lizenz (Deutsch): | Zweitveröffentlichung |