A low-cost automized anaerobic chamber for long-term growth experiments and sample handling

  • The handling of oxygen sensitive samples and growth of obligate anaerobic organisms requires the stringent exclusion of oxygen, which is omnipresent in our normal atmospheric environment. Anaerobic workstations (aka. Glove boxes) enable the handling of oxygen sensitive samples during complex procedures, or the long-term incubation of anaerobic organisms. Depending on the application requirements, commercial workstations can cost up to 60.000 €. Here we present the complete build instructions for a highly adaptive, Arduino based, anaerobic workstation for microbial cultivation and sample handling, with features normally found only in high cost commercial solutions. This build can automatically regulate humidity, H2 levels (as oxygen reductant), log the environmental data and purge the airlock. It is built as compact as possible to allow it to fit into regular growth chambers for full environmental control. In our experiments, oxygen levels during the continuous growth of oxygen producing cyanobacteria, stayed under 0.03 % for 21 days without needing user intervention. The modular Arduino controller allows for the easy incorporation of additional regulation parameters, such as CO2 concentration or air pressure. This paper provides researchers with a low cost, entry level workstation for anaerobic sample handling with the flexibility to match their specific experimental needs.

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Author:Achim Herrmann, Michelle Gehringer
Parent Title (English):HardwareX
Document Type:Article
Language of publication:English
Publication Date:2021/10/05
Year of Publication:2021
Publishing Institute:Technische Universität Kaiserslautern
Date of the Publication (Server):2021/12/21
Issue:10, 2021, Article e00236
Number of page:13
Faculties / Organisational entities:Fachbereich Biologie
DDC-Cassification:5 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Licence (German):Zweitveröffentlichung