Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter

  • A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from Escherichia coli.

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Author:Sarah Di Nonno, Roland Ulber
URN:urn:nbn:de:hbz:386-kluedo-70241
Parent Title (English):Sensors
Publisher:MDPI
Document Type:Article
Language of publication:English
Date of Publication (online):2022/10/18
Year of first Publication:2022
Publishing Institution:Technische Universität Kaiserslautern
Date of the Publication (Server):2022/12/02
Issue:2022, 22, 7916
Page Number:13
Source:https://doi.org/10.3390/s22207916
Faculties / Organisational entities:Kaiserslautern - Fachbereich Maschinenbau und Verfahrenstechnik
DDC-Cassification:6 Technik, Medizin, angewandte Wissenschaften / 620 Ingenieurwissenschaften und Maschinenbau
Collections:Open-Access-Publikationsfonds
Licence (German):Zweitveröffentlichung