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Polyphenolic composition of extracts from winery by-products and effects on cellular cytotoxicity and mitochondrial functions in HepG2 cells

  • Winery by-products arise in high amounts during winemaking processes. Hence, recovery alternatives are of great interest. In this study, effects of extracts from winery by-products (Vitis vinifera L. cv. Riesling) on mitochondrial functions in human hepatocellular carcinoma (HepG2) cells were examined. Polyphenolic profiles of pomace (PE), stem (SE), vine leaf (VLE), and vine shoot extracts (VSE) were characterized by HPLC-UV/Vis-ESI-MS/MS. The extracts induced dose-dependent cytotoxic effects (PE > SE > VLE > VSE). VSE showed protective effects regarding modulation of tert-butyl hydroperoxide (TBH)-induced intracellular reactive oxygen species (ROS) levels. PE, SE and VLE increased the mitochondrial membrane potential (MMP), whereas VSE decreased it owing to mildly impaired mitochondrial respiration. Cells may try to compensate reduced respiration chain complex activities by increasing the mitochondrial mass, as indicated by enhanced citrate synthase activity and mRNA expression levels after VSE incubation. Thus, winery by-products represent interesting sources of bioactive compounds that exert positive or negative effects on mitochondrial functions.
Author:Christine Fuchs, Tamara Bakuradze, Regina Steinke, Rekha Grewal, Gunter P. Eckert, Elke RichlingORCiD
Parent Title (English):Journal of Functional Foods
Document Type:Article
Language of publication:English
Publication Date:2020/05/12
Year of Publication:2020
Publishing Institute:Technische Universität Kaiserslautern
Date of the Publication (Server):2020/09/09
Issue:70 (2020)
Number of page:14
Faculties / Organisational entities:Fachbereich Chemie
DDC-Cassification:5 Naturwissenschaften und Mathematik / 540 Chemie
Licence (German):Zweitveröffentlichung